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sodium dodecyl binding

sodium dodecyl binding

  • Sodium Dodecyl Sulfate - an overview | ScienceDirect Topics

    2Sodium dodecyl sulfate (SDS), also known as lauryl sulfate, is an ionic detergent that is useful for the rapid disruption of biological membranes. It is a key component of many reagents used to purify nucleic acids because of its abilities to quickly disrupt the tissue architecture and to inhibit both RNase and deoxyribonuclease (DNase) activity.

  • The binding of sodium dodecyl sulphate to various proteins

    21. The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. 2. Most of the proteins studied bound 90–100% of their weight of sodium dodecyl sulphate. 3. The glycoproteins studied bound 70–100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of the molecule. 4.

  • binding of sodium dodecyl sulphate to various proteins ...

    21. The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. 2. Most of the proteins studied bound 90–100% of their weight of sodium dodecyl sulphate. 3. The glycoproteins studied bound 70–100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of...

  • Binding of Sodium Dodecyl Sulfate with Linear and Branched ...

    2Isothermal titration microcalorimetry (ITC), conductivity, and turbidity measurements have been carried out to study the interaction of sodium dodecyl sulfate (SDS) with polyethyleneimines (PEI) including linear PEI and branched PEI at different pH values of 3, 7, and 10. In all cases, the s show a remarkable affinity toward SDS.

  • Binding of Dodecyl Sulfate to Proteins at High Binding Ratios ...

    2A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature.

  • The Binding of Detergents to Proteins

    2The binding of sodium dodecyl sulfate to proteins has been studied since the mid-1940’s (3). A large body of work has ap- peared since then and is summarized in Reference 4. Further- more, two groups have already studied the binding of very high

  • Study of the binding of sodium dodecylsulfate to dibasic ...

    2SDS strongly binds to dibasic amino acids; the relative strength of interaction is significantly higher with Lys and Orn than with Arg. These results indicate that SDS probably binds to the secondary amino groups of Lys in proteins. The impact of sodium chloride and acetic acid was similar, but the effect of sodium acetate was significantly weaker.

  • Beyond the detergent effect: a binding site for sodium ...

    2The dodecyl chain of the detergent penetrates into the hydrophobic cavity, bending into a horseshoe shape to conform to the shape of the binding cavity. The cavity lies directly on a crystallographic twofold axis of symmetry that relates two apoferritin protomers, both of which contribute residues that form the ligand-binding cavity.

  • Sodium dodecyl sulfate - Wikipedia

    2Sodium dodecyl sulfate (SDS), synonymously, sodium lauryl sulfate (SLS), or sodium laurilsulfate, is a synthetic organic compound with the formula C H 3 (CH 2) 11 SO 4 Na. It is an anionic surfactant used in many cleaning and hygiene products. The sodium salt is of an organosulfate class of organics.

  • SDS-PAGE - Wikipedia

    2SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. It uses sodium dodecyl sulfate (SDS) molecules to help identify and isolate protein molecules.

  • Binding of Dodecyl Sulfate to Proteins at High Binding Ratios ...

    2A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature.

  • Binding of Sodium Dodecyl Sulfate to Some Polyethyleneimines ...

    2Binding of Sodium Dodecyl Sulfate to Linear and Star Homo s of the Nonionic Poly(methoxyhexa(ethylene glycol) methacrylate) and the Polycation Poly(2-(dimethylamino)ethyl methacrylate): Electromotive Force, Isothermal Titration Calorimetry, Surface Tension, and Small-Angle Neutron Scattering Measurements.

  • The binding of sodium dodecyl sulphate to various proteins ...

    21. The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. 2. Most of the proteins studied bound 90–100% of their weight of sodium dodecyl sulphate. 3. The glycoproteins studied bound 70–100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of the molecule. 4.

  • Bindingof Dodecyl SulfatetoProteinsat High Binding Ratios ...

    2Binding ratios for bovine serum albumin andhemoglobin were also obtained bydialyz-ing the native protein against SDSand beta-mercaptoethanol thus omitting the GuHCl step. Sodium dodecyl sulfate concentrations in the dialysate and the protein solution were determinedbyextraction of analkyl sulfate-methylene blue complexinto chloroformand

  • Beyond the detergent effect: a binding site for sodium ...

    2Although sodium dodecyl sulfate (SDS) is widely used as an anionic detergent, it can also exert specific pharmacological effects that are independent of the surfactant properties of the molecule. However, structural details of how proteins recognize SDS are scarce.

  • What does sodium dodecyl sulfate do? | AnswersDrive

    2Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight.

  • Effect of quaternary ammonium substitution of ...

    2The binding behavior of sodium dodecyl sulfate (SDS) to quater-nary ammonium substituted hydroxyethylcellulose (cationic cellu-lose) of varying degree of substitution was studied by the following measurements: binding isotherms of SDS to cationic cellulose, dye solubilization of the cationic cellulose/SDS complex, partial molal volume of each cationic cellulose, and spin-lattice relaxation time of SDS protons in the course of binding.

  • Binding of Sodium Dodecyl Sulfate to a Cationic of ...

    2Binding of Sodium Dodecyl Sulfate to a Cationic of High Charge Density. The intrinsic binding constant (K) and the critical aggregation concentration (cac) decreased and then increased with increasing temperature, having minimum values of 17.9 x 10 (-2) mM (-1) and 0.19 x 10 (-2) mM at 25 degreesC, respectively.

  • Capillary Electrophoresis–Sodium Dodecyl Sulfate of a Heavily ...

    2In the biotechnology industry, traditional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has been widely used to monitor the integrity and purity of therapeutic proteins during formulation and process development, and for lot release and stability testing.

  • What are the functions of SDS in DNA extraction? - Quora

    2SDS stands for 'sodium dodecyl sulfate' is a solid anionic detergent that can solubilize the proteins and lipids that frame the membranes. This will help the cell membranes to separate and expose the chromosomes that contain the DNA. SDS release the DNA from histones and other DNA binding proteins by denaturing them. It has negative charge.

  • The Molecular Basis of the Sodium Dodecyl Sulfate Effect on ...

    2To investigate the molecular interactions of sodium dodecyl sulfate (SDS) with human ubiquitin and its unfolding mechanisms, a comparative study was conducted on the interactions of the protein in ...

  • What is the function of sodium dodecyl sulfate? | AnswersDrive

    2SDS stands for 'sodium dodecyl sulfate' is a solid anionic detergent that can solubilize the proteins and lipids that frame the membranes. This will help the cell membranes to separate and expose the chromosomes that contain the DNA. SDS release the DNA from histones and other DNA binding proteins by denaturing them.

  • "A Single Amino Acid Substitution in a Histidine-Transport ...

    2This arginine residue is therefore potentially essential for the transport function of the protein. The mutant protein migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis more slowly than the wild type protein, as if its molecular weight were greater by as much as 2000.

  • Sulfate - Columbia University

    2Binding of Sodium DodecyI Sulfate to Polyethylene Oxide at the Silica-Water Interface. The effect of prC'adsorbed sx>lethylene oxide ( PEO) on silica on the subsequent adsorptioo of sodium dodec)1 sulfate (50s) on the silica was investipted in this study.

  • Product Name Sodium lauryl sulfate | Sigma-Aldrich

    2Sodium n-Dodecyl Sulfate, High Purity - CAS 151-21-3 - Calbiochem. ... Inhibits bacterial protein synthesis by binding to the 23S RNA in 50S ribosome. Millipore

  • Interactions between gelatin and sodium dodecyl sulphate ...

    2The binding of sodium dodecyl sulfate (SDS) and sodium octyl sulfate (SOS) to gelatin and its derivatives at pH 5. 80 has been studied by the method of equilibrium dialysis.

  • Sodium dodecyl sulfate - Alchetron, The Free Social Encyclopedia

    2Sodium dodecyl sulfate, synonymously sodium lauryl sulfate (or laurilsulfate; SDS or SLS, respectively), is a synthetic organic compound with the formula CH 3 (CH 2) 11 SO 4 Na. It is an anionic surfactant used in many cleaning and hygiene products. The sodium salt is of an organosulfate class of organics.

  • Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis ...

    2SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry and molecular biology to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular weight as well as higher order protein folding, posttranslational modifications and other factors). 1

  • The Gross Conformation of Protein-Sodium Dodecyl Sulfate ...

    2The interaction of sodium dodecyl sulfate with a wide variety of proteins is characterized by a high binding ratio when the monomer concentration of amphiphile exceeds 5 x 10W4 M. This binding ratio on a gram to gram basis is identi- cal for all proteins investigated. The protein portion of the

  • A Rapid and Sensitive Method for the titation of ...

    2sodium dodecyl sulfate, Triton X-100, and commercial glassware detergents. Interference by small amounts of detergent may be eliminated by the use of proper controls. Laboratory practice in protein purification often requires a rapid and sensitive method for the titation of protein. Methods presently

  • Sodium dodecyl sulfate (SDS) 10% in aqueous solution ...

    2Sodium dodecyl sulfate or sodium lauryl sulfate (SDS/SLS, respectively) is an anionic surfactant commonly used in polyacrylamide gel electrophoresis for the determination of protein molecular weight. In most proteins, the binding of SDS to the polypeptide chain imparts an even distribution of charge per unit mass, thereby resulting in a ...

  • The Molecular Basis of the Sodium Dodecyl Sulfate Effect on ...

    2To investigate the molecular interactions of sodium dodecyl sulfate (SDS) with human ubiquitin and its unfolding mechanisms, a comparative study was conducted on the interactions of the protein in ...

  • What is the function of sodium dodecyl sulfate? | AnswersDrive

    2Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight.

  • Binding of Sodium Dodecyl Sulfate to Some Polyethyleneimines ...

    2Binding of Sodium Dodecyl Sulfate to Some Polyethyleneimines and Their Ethoxylated Derivatives at Different pH Values. Electromotive Force and Microcalorimetry Studies @inproceedings{Li2000BindingOS, title={Binding of Sodium Dodecyl Sulfate to Some Polyethyleneimines and Their Ethoxylated Derivatives at Different pH Values.

  • Sodium dodecyl sulphate - Substance Information - ECHA

    2Sodium dodecyl sulphate. Danger! According to the classification provided by companies to ECHA in REACH registrations this substance is harmful if swallowed, causes serious eye damage, is harmful to aquatic life with long lasting effects, is a flammable solid, is harmful if inhaled, causes skin irritation and may cause respiratory irritation.

  • Interaction of sodium dodecyl sulfate with polyethyleneimine ...

    2The binding isotherm of sodium dodecyl sulfate (SDS) on a hyperbranched polyethyleneimine (PEI) was determined by an equilibrium dialysis method. Dynamic light scattering, electrophoretic mobility, and coagulation kinetics measurements were also performed in order to monitor the changes in the charged nature and size of PEI/SDS complexes.

  • Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis ...

    2SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry and molecular biology to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular weight as well as higher order protein folding, posttranslational modifications and other factors). 1

  • Detection of Calcium Binding Proteins by 45 Ca ...

    2Two high molecular weight calcium binding proteins were present in the scallop striated muscle. The procedure described can be completed within 24 h and can detect as little as 2 μg of calcium binding protein in the starting sample.

  • Effects of sodium dodecyl sulfate of polyphenoloxidase ...

    2Sodium dodecyl sulfate (SDS), adsorbed from aqueous solution onto a monolayer of dimethyloctadecylsiloxane (C[sub 15]) bonded to silica, was characterized spectroscopically. The structural and dynamical properties of the adsorbed SDS were probed by the fluorescence anisotropy of a dilute hydrophobic fluorophor, 1,4-bis(o-methylstyryl)benzene.

  • Effects of Sodium Dodecyl Sulfate, 2-Mercaptoethanol and EDTA ...

    2The effects of sodium dodecyl sulfate, 2-mercaptoethanol and EDTA on the distribution and binding status of copper and zinc in the soybean protein fraction were examined by gel filtration on Sephadex G-100. Copper and zinc in the protein fraction were both associated primarily with the major components of >100, 000 in mol wt.